Recombinant Mouse G-CSF Protein

Name: Recombinant Mouse G-CSF Protein
Brand: Novus Biologicals
SKU: NBP2-35061

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35061

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Product Specifications
Scientific Data
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Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

P09920

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all G-CSF Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 178 amino acids corresponding to G-CSF Source: E. coli

Uniprot ID: P09920

Amino Acid Sequence: VPLVTVSALP PSLPLPRSFL LKSLEQVRKI QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC SSQALQQTQC LSQLHSGLCL YQGLLQALSG ISPALAPTLD LLQLDVANFA TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF QRRAGGVLAI SYLQGFLETA RLALHHLA

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of G-CSF as determined by LAL method.

Predicted Molecular Mass

18.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

G-CSF protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x 10^7 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse G-CSF Protein

SDS-PAGE: Recombinant Mouse G-CSF Protein [NBP2-35061]

SDS-Page: Mouse G-CSF Protein [NBP2-35061]

Formulation, Preparation and Storage

NBP2-35061

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 10 mM Sodium Citrate, pH 4.0, 150 mM NaCl, 0.01 % Tween-20.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: G-CSF

Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Besides, many other cell types can secreted this protein after LPS, IL-1 or TNF- alpha activation, which are fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF is cytokine that acts in hematopoiesis by controlling the production, differentiation and function of 2 related white cell populations of the blood, the granulocytes and the monocytes- macrophages. In addition, it may function in some adhesion or recognition events at the cell surface. The murine G-CSF cDNA encodes a 208 amino acid (a.a.) residue precursor protein containing a 30 a.a. residue signal peptide that is proteolytically cleaved to generate the 178 a.a. residue mature protein. Murine G-CSF is 73 % identical at the amino acid level to human G-CSF and the two proteins show species cross-reactivity.

Long Name

Granulocyte Colony Stimulating Factor

Alternate Names

C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin

Gene Symbol

CSF3

UniProt