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Recombinant Human GADD45 alpha His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44475

Novus Biologicals, part of Bio-Techne
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NBP1-44475

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-165 of Human GADD45 alpha

Source: E.coli

Amino Acid Sequence: MTLEEFSAGE QKTERMDKVG DALEEVLSKA LSQRTITVGV YEAAKLLNVD PDNVVLCLLA ADEDDDRDVA LQIHFTLIQA FCCENDINIL RVSNPGRLAE LLLLETDAGP AASEGAEQPP DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPERLEHHH HHH

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

19.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GADD45 alpha His Protein

SDS-PAGE: Recombinant Human GADD45 alpha His Protein [NBP1-44475]

SDS-PAGE: Recombinant Human GADD45 alpha His Protein [NBP1-44475]

SDS-Page: Recombinant Human GADD45 alpha Protein [NBP1-44475] - GADD45A, 19.4 kDa (173aa) with a purity of 85% by SDS - PAGE

Formulation, Preparation and Storage

NBP1-44475
Formulation 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1 M NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GADD45 alpha

GADD45A, also known as growth arrest and DNA damage-inducible protein, binds both Cdks and PCNA, a protein involved in DNA replication and repair. It has been shown to stimulate DNA excision repair in vitro and to inhibit entry of cells into S phase. This protein may serve as a link between p53-dependent cell cycle checkpoint and DNA repair. Recombinant human GADD45A protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Growth Arrest and DNA Damage Gene alpha

Alternate Names

GADD45a

Gene Symbol

GADD45A

Additional GADD45 alpha Products

Product Documents for Recombinant Human GADD45 alpha His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human GADD45 alpha His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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