Recombinant Human Galectin-3 Protein

Name: Recombinant Human Galectin-3 Protein
Brand: Novus Biologicals
SKU: NBP2-34881

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34881

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Product Datasheet / COA

Catalog #

Availability

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100% Guarantee

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P17931

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Galectin-3 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 249 amino acids corresponding to Galectin-3 Source: E. coli

Uniprot ID: P17931

Amino Acid Sequence: ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYPGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSATGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Galectin-3 as determined by LAL method.

Predicted Molecular Mass

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Galectin-3 protein is fully biologically active when compared to standard. The ED50 as determined by its ability to agglutinate human red blood cells is less than 10 ug/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Galectin-3 Protein

SDS-PAGE: Recombinant Human Galectin-3 Protein [NBP2-34881]

SDS-Page: Human Galectin-3 Protein [NBP2-34881]

Formulation, Preparation and Storage

NBP2-34881

Formulation	Lyophilized from a 0.2 um filtered solution in 1xPBS, 0.02% Tween-20, 3mM beta-ME, pH 7.4.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Galectin-3

Human Galectin-3 also named AGE-R3, CBP35, GAL3, L29, LGALS3, Mac-2, is belonging to the galectins family and it is encoded by a single gene, LGALS3, located on chromosome 14, locus q21-q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Galectin-3 is approximately 30 kDa and, like all galectins, contains a carbohydrate-recognition-binding domain (CRD) of about 130 amino acids that enable the specific binding of Beta-galactosides. Given Galectin-3's broad biological functionality, it has been demonstrated to be involved in cancer, inflammation and fibrosis, heart disease, and stroke. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Human Galectin-3 shares 79% amino acid sequence identity with rat and mouse Galectin-3, respectively.

Alternate Names

AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2

Gene Symbol

LGALS3

UniProt

P17931 (Mouse)

Additional Galectin-3 Products

Product Documents for Recombinant Human Galectin-3 Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human Galectin-3 Protein

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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