Recombinant Human GALT His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23068

Novus Biologicals, part of Bio-Techne
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NBP2-23068
NBP2-23068-0.25mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all GALT Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-379 of Human GALT

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSRSGT DPQQRQQASE ADAAAATFRA NDHQHIRYNP LQDEWVLVSA HRMKRPWQGQ VEPQLLKTVP RHDPLNPLCP GAIRANGEVN PQYDSTFLFD NDFPALQPDA PSPGPSDHPL FQAKSARGVC KVMCFHPWSD VTLPLMSVPE IRAVVDAWAS VTEELGAQYP WVQIFENKGA MMGCSNPHPH CQVWASSFLP DIAQREERSQ QAYKSQHGEP LLMEYSRQEL LRKERLVLTS EHWLVLVPFW ATWPYQTLLL PRRHVRRLPE LTPAERDDLA SIMKKLLTKY DNLFETSFPY SMGWHGAPTG SEAGANWNHW QLHAHYYPPL LRSATVRKFM VGYEMLAQAQ RDLTPEQAAE RLRALPEVHY HLGQKDRETA TIA

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

45.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GALT His Protein

SDS-PAGE: Recombinant Human GALT His Protein [NBP2-23068]

SDS-PAGE: Recombinant Human GALT His Protein [NBP2-23068]

SDS-Page: GALT Protein [NBP2-23068]

Formulation, Preparation and Storage

NBP2-23068
Formulation 20 mM Tris-HCl buffer (pH 8.5), 0.2 M NaCl, 10% glycerol
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GALT

GALT catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human GALT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Alternate Names

EC 2.7.7.12, Gal-1-P uridylyltransferase, galactose-1-phosphate uridyl transferase, galactose-1-phosphate uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase

Gene Symbol

GALT

Additional GALT Products

Product Documents for Recombinant Human GALT His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human GALT His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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