At which residues of NBP1-72534 is the His-tag located?

Name: Recombinant Human gamma Tubulin His Protein
Brand: Novus Biologicals
SKU: NBP1-72534

MGSSHHHHHH SSGLVPRGSH MPREIITLQL GQCGNQIGFE....... MPRE is the start of the protein sequence.

Recombinant Human gamma Tubulin His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72534

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all gamma Tubulin Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-451 of Human gamma Tubulin

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPREIITLQL GQCGNQIGFE FWKQLCAEHG ISPEGIVEEF ATEGTDRKDV FFYQADDEHY IPRAVLLDLE PRVIHSILNS PYAKLYNPEN IYLSEHGGGA GNNWASGFSQ GEKIHEDIFD IIDREADGSD SLEGFVLCHS IAGGTGSGLG SYLLERLNDR YPKKLVQTYS VFPNQDEMSD VVVQPYNSLL TLKRLTQNAD CVVVLDNTAL NRIATDRLHI QNPSFSQINQ LVSTIMSAST TTLRYPGYMN NDLIGLIASL IPTPRLHFLM TGYTPLTTDQ SVASVRKTTV LDVMRRLLQP KNVMVSTGRD RQTNHCYIAI LNIIQGEVDP TQVHKSLQRI RERKLANFIP WGPASIQVAL SRKSPYLPSA HRVSGLMMAN HTSISSLFER TCRQYDKLRK REAFLEQFRK EDMFKDNFDE MDTSREIVQQ LIDEYHAATR PDYISWGTQE Q

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

53.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human gamma Tubulin His Protein

SDS-PAGE: Recombinant Human gamma Tubulin His Protein [NBP1-72534]

SDS-Page: Recombinant Human gamma Tubulin Protein [NBP1-72534] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation and Storage

NBP1-72534

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 M UREA, 5% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: gamma Tubulin

TUBG1 is a member of the tubulin superfamily. This protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. It mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. Recombinant human TUBG1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

Tubulin gamma-1 chain

Alternate Names

Gamma-1-tubulin, GCP-1, TUBG, TUBG1

Gene Symbol

TUBG1

Additional gamma Tubulin Products

Product Documents for Recombinant Human gamma Tubulin His Protein

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COA

Product Specific Notices for Recombinant Human gamma Tubulin His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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