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Recombinant Mouse GDF-5/BMP-14 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61339

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-61339-100ug
NBP2-61339-10ug
NBP2-61339-1mg
NBP2-61339-500ug

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Description

A disulfide-linked homodimeric protein containing two 120 amino acids chains corresponding to GDF-5/BMP-14 Source: E. coli

Uniprot ID: P43027

Amino Acid Sequence: APLANRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of GDF-5/BMP-14 as determined by LAL method.

Predicted Molecular Mass

27.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 ug/ml, corresponding to a specific activity of > 1000 IU/mg.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-61339
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: GDF-5/BMP-14

The GDF5 gene is involved in skeletal development through bone and cartilage formation. This gene encodes for a 501 amino acid long, 55 kDA protein that is part of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily which is known to regulate cell growth and differentiation in embryotic and adult tissues. The GDF5 gene is involved in paxillian interactions, mitochondrial apoptosis, the cytokine-cytokine receptor interaction, and the TGF-beta signaling pathway through interacting with other genes such as BMPR1A, BMPR1B, BMPR2, HTRA1, and CHRDL2. The GDF5 gene has been associated to chondrodysplasia, synostosis, periostitis, short stature, cushing's symphalangism, brachydactyly and ankylosis.

Long Name

Growth Differentiation Factor 5

Alternate Names

BMP-14, CDMP-1, GDF5

Gene Symbol

GDF5

Additional GDF-5/BMP-14 Products

Product Documents for Recombinant Mouse GDF-5/BMP-14 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Mouse GDF-5/BMP-14 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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