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Recombinant Human GDF-6/BMP-13 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61325

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-61325-100ug
NBP2-61325-10ug
NBP2-61325-1mg
NBP2-61325-500ug

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Description

A disulfide-linked homodimeric protein containing two 120 amino acid chains corresponding to GDF-6/BMP-13 Source: E. coli

Uniprot ID: Q6KF10

Amino Acid Sequence: TAFASRHGKR HGKKSRLRCS KKPLHVNFKE LGWDDWIIAP LEYEAYHCEG VCDFPLRSHL EPTNHAIIQT LMNSMDPGST PPSCCVPTKL TPISILYIDA GNNVVYKQYE DMVVESCGCR

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of GDF-6/BMP-13 as determined by LAL method.

Predicted Molecular Mass

27.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 2.0 ug/ml, corresponding to a specific activity of > 500 IU/mg.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-61325
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: GDF-6/BMP-13

GDF6 encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily of secreted signaling molecules. It is required for normal formation of some bones and joints in the limbs, skull, and axial skeleton. Mutations in this gene result in colobomata, which are congenital abnormalities in ocular development, and in Klippel-Feil syndrome (KFS), which is a congenital disorder of spinal segmentation. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Long Name

Growth Differentiation Factor 6

Alternate Names

BMP-13, GDF6

Gene Symbol

GDF6

Additional GDF-6/BMP-13 Products

Product Documents for Recombinant Human GDF-6/BMP-13 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human GDF-6/BMP-13 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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