Recombinant Human GDNF Protein

Name: Recombinant Human GDNF Protein
Brand: Novus Biologicals
SKU: NBP2-34888

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34888

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P39905

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all GDNF Proteins and Enzymes »

Product Specifications

Description

A homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains corresponding to GDNF Source: E. coli

Uniprot ID: P39905

Amino Acid Sequence: SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCRPIAFDDD LSFLDDNLVY HILRKHSAKR CGCI

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of GDNF as determined by LAL method.

Predicted Molecular Mass

30.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 5.0 x 10^7 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GDNF Protein

SDS-PAGE: Recombinant Human GDNF Protein [NBP2-34888]

SDS-Page: Human GDNF Protein [NBP2-34888]

Formulation, Preparation and Storage

NBP2-34888

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, pH 7.4, with 0.05 % Tween-20.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: GDNF

Glial cell-derived neurotrophic factor is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. It is a founding member of the GDNF family of ligands (GFL) and has been shown to interact with GFRA2 and GDNF family receptor alpha. GDNF (monomer) contains seven conserved cysteine residues, one of which (Cys 101) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration. Rat and human mature GDNF shows 93 % sequence identity and have species cross-reactivity.

Long Name

Glial Cell line-derived Growth Factor

Alternate Names

ATF, HFB1-GDNF, HGDNF, HSCR3

Gene Symbol

GDNF

UniProt

P39905 (Goat)

Additional GDNF Products

Product Documents for Recombinant Human GDNF Protein

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COA

Product Specific Notices for Recombinant Human GDNF Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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