Recombinant Human GILT/IFI30 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23146

Novus Biologicals, part of Bio-Techne
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NBP2-23146
NBP2-23146-0.25mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all GILT/IFI30 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 58-232 of Human GILT/IFI30

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNAPLVN VTLYYEALCG GCRAFLIREL FPTWLLVMEI LNVTLVPYGN AQEQNVSGRW EFKCQHGEEE CKFNKVEACV LDELDMELAF LTIVCMEEFE DMERSLPLCL QLYAPGLSPD TIMECAMGDR GMQLMHANAQ RTDALQPPHE YVPWVTVNGK PLEDQTQLLT LVCQLYQGK

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GILT/IFI30 His Protein

SDS-PAGE: Recombinant Human GILT/IFI30 His Protein [NBP2-23146]

SDS-PAGE: Recombinant Human GILT/IFI30 His Protein [NBP2-23146]

SDS-Page: Recombinant Human GILT/IFI30 Protein [NBP2-23146] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. Molecular Weight: 22.5 kDa (199aa) confirmed by MALDI-TOF

Formulation, Preparation and Storage

NBP2-23146
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 10% glycerol, 1 mM DTT
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GILT/IFI30

IFI30, also as known as gamma-interferon-inducible lysosomal thiol reductase, belongs to the GILT family. This protein is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. This protein facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or cross-presentation. Recombinant human IFI30 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Long Name

Gamma-Interferon-inducible Lysosomal Thiol Reductase

Alternate Names

IFI30, IP30, Legumaturain

Gene Symbol

IFI30

Additional GILT/IFI30 Products

Product Documents for Recombinant Human GILT/IFI30 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human GILT/IFI30 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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