We recently purchased Recombinant Human TIM-3 Protein (NBP2-23118) and GITR/TNFRSF18 ( NBP2-52215-0.05mg). Can these be diluted with sterile Di water without harming the integrity/functionality of the proteins?

Name: Recombinant Human GITR/TNFRSF18 His Protein
Brand: Novus Biologicals
SKU: NBP2-52215

Recombinant Human TIM-3 Protein (NBP2-23118) and Recombinant Human GITR/TNFRSF18 Protein (NBP2-52215) have been tested in PAGE but we have not tested its bioactivity/functional aspects so far. The buffer for NBP2-23118 (20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol) contains urea and glycerol, which indicates there is the solubility/aggregation issue at the high concentration. I anticipate that if diluting with sterile double distilled water, at the certain concentrations, the protein will not be stable and precipitated out. Unfortunately, our lab did not measure the thresholds for the precipitations. On the other hand, NBP2-52215 has phosphate buffered saline (pH 7.4) and 10% glycerol as the buffer, which maybe tolerable in the water dilution process; however we have not done so in the past.

Recombinant Human GITR/TNFRSF18 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52215

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Baculovirus

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all GITR/TNFRSF18 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 26-162 of Human GITR/TNFRSF18

Source:Baculovirus

Amino Acid Sequence: QRPTGGPGCG PGRLLLGTGT DARCCRVHTT RCCRDYPGEE CCSEWDCMCV QPEFHCGDPC CTTCRHHPCP PGQGVQSQGK FSFGFQCIDC ASGTFSGGHE GHCKPWTDCT QFGFLTVFPG NKTHNAVCVP GSPPAEPLEH HHHHH

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

15.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GITR/TNFRSF18 His Protein

SDS-PAGE: Recombinant Human GITR/TNFRSF18 His Protein [NBP2-52215]

SDS-Page: Recombinant Human GITR/TNFRSF18 Protein [NBP2-52215]

Formulation, Preparation and Storage

NBP2-52215

Formulation	PBS (pH 7.4), 10% glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GITR/TNFRSF18

GITR is encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]

Long Name

Glucocorticoid Induced TNF Receptor Family Related Gene

Alternate Names

AITR, CD357, TNFRSF18

Gene Symbol

TNFRSF18

Additional GITR/TNFRSF18 Products

Product Documents for Recombinant Human GITR/TNFRSF18 His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human GITR/TNFRSF18 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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