Recombinant E. coli Glucose 6 Phosphate Dehydrogenase Protein

Name: Recombinant E. coli Glucose 6 Phosphate Dehydrogenase Protein
Brand: Novus Biologicals
SKU: NBC1-19064

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-19064

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Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all G6PD Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids1-491 of E.coli Glucose 6 Phosphate Dehydrogenase

Source: E.coli

Amino Acid Sequence: MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

55.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 50 units/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli Glucose 6 Phosphate Dehydrogenase Protein

Western Blot: Recombinant E. coli Glucose 6 Phosphate Dehydrogenase Protein [NBC1-19064]

Western Blot: Glucose 6 Phosphate Dehydrogenase Protein [NBC1-19064] - 55.7kDa (491 aa) on SDS - PAGE

Formulation, Preparation and Storage

NBC1-19064

Formulation	PBS (pH 7.4), 10% glycerol, 0.1 mM PMSF, 2 mM EDTA, 0.5 MM DTT
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: G6PD

Glucose 6 Phosphate Dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant G6PD protein was expressed in E.coli and purified by conventional chromatography techniques.

Long Name

Glucose-6-Phosphate Dehydrogenase

Alternate Names

G6PDH

Gene Symbol

G6PD

Additional G6PD Products

Product Documents for Recombinant E. coli Glucose 6 Phosphate Dehydrogenase Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant E. coli Glucose 6 Phosphate Dehydrogenase Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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