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Recombinant E. coli Glutaminase His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72301

Novus Biologicals, part of Bio-Techne
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NBP1-72301-0.1mg
NBP1-72301-0.5mg

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-310 of E.coli Glutaminase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MLDANKLQQA VDQAYTQFHS LNGGQNADYI PFLANVPGQL AAVAIVTCDG NVYSAGDSDY RFALESISKV CTLALALEDV GPQAVQDKIG ADPTGLPFNS VIALELHGGK PLSPLVNAGA IATTSLINAE NVEQRWQRIL HIQQQLAGEQ VALSDEVNQS EQTTNFHNRA IAWLLYSAGY LYCDAMEACD VYTRQCSTLL NTIELATLGA TLAAGGVNPL THKRVLQADN VPYILAEMMM EGLYGRSGDW AYRVGLPGKS GVGGGILAVV PGVMGIAAFS PPLDEDGNSV RGQKMVASVA KQLGYNVFKG

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli Glutaminase His Protein

SDS-PAGE: Recombinant E. coli Glutaminase His Protein [NBP1-72301]

SDS-PAGE: Recombinant E. coli Glutaminase His Protein [NBP1-72301]

SDS-Page: Recombinant E. coli Glutaminase Protein [NBP1-72301] - 15% SDS-Page (3ug)

Formulation, Preparation and Storage

NBP1-72301
Formulation 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, 100 mM NaCl
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glutaminase

GlsA1 (glutaminase1) belongs to the large superfamily of serine-dependent beta-lactamases and penicillin-binding proteins. They are present in most bacteria and eukaryotes and catalyze the hydrolytic deamidation of l-glutamine to l-glutamate and free NH4+. Recombinant E.coli glsA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Glutaminase Kidney Isoform, Mitochondrial

Alternate Names

GLS, K-Glutaminase, L-Glutamine Amidohydrolase

Gene Symbol

GLS

Additional Glutaminase Products

Product Documents for Recombinant E. coli Glutaminase His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant E. coli Glutaminase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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