Recombinant Human Glycerol 3 Phosphate Dehydrogenase Protein

Name: Recombinant Human Glycerol 3 Phosphate Dehydrogenase Protein
Brand: Novus Biologicals
SKU: NBP1-40405

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-40405

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Glycerol 3 Phosphate Dehydrogenase Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 1-349 of Human Glycerol 3 Phosphate Dehydrogenase

Source: E.coli

Amino Acid Sequence: MASKKVCIVG SGNWGSAIAK IVGGNAAQLA QFDPRVTMWV FEEDIGGKKL TEIINTQHEN VKYLPGHKLP PNVVAVPDVV QAAEDADILI FVVPHQFIGK ICDQLKGHLK ANATGISLIK GVDEGPNGLK LISEVIGERL GIPMSVLMGA NIASEVADEK FCETTIGCKD PAQGQLLKEL MQTPNFRITV VQEVDTVEIC GALKNVVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK LFCSGPVSSA TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE LLNGQKLQGP ETARELYSIL QHKGLVDKFP LFMAVYKVCY EGQPVGEFIH CLQNHPEHM

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

37.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Glycerol 3 Phosphate Dehydrogenase Protein

SDS-PAGE: Recombinant Human Glycerol 3 Phosphate Dehydrogenase Protein [NBP1-40405]

SDS-Page: Recombinant Human Glycerol 3 Phosphate Dehydrogenase Protein [NBP1-40405] - GPD1, 37.5 kDa (349aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Formulation, Preparation and Storage

NBP1-40405

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glycerol 3 Phosphate Dehydrogenase

GPD1, also known as glycerol-3-phosphate dehydrogenase, is an enzyme that catalyzes the reduction of dihydroxyacetone phosphate to sn-glycerol 3-phosphate. (sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH) Older terms for glycerol-3-phosphate dehydrogenase include alpha glycerol-3-phosphate dehydrogenase and glycerolphosphate dehydrogenase. However, GPD1 is not the same as glyceraldehyde 3-phosphate dehydrogenase (GAPDH) whose substrate is an aldehyde not an alcohol. Recombinant GPD1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

EC 1.1.1, EC 1.1.1.8, FLJ26652, glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic, glycerol-3-phosphate dehydrogenase 1 (soluble), glycerol-3-phosphate dehydrogenase, soluble, GPD-C, GPDH-C

Gene Symbol

GPD1

Additional Glycerol 3 Phosphate Dehydrogenase Products

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COA

Product Specific Notices for Recombinant Human Glycerol 3 Phosphate Dehydrogenase Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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