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Recombinant Rat GM-CSF Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35206

Novus Biologicals, part of Bio-Techne
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NBP2-35206-100ug
NBP2-35206-1mg
NBP2-35206-500ug
NBP2-35206-5ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 127 amino acids corresponding to GM-CSF Source: E. coli

Uniprot ID: P48750

Amino Acid Sequence: APTRSPNPVT RPWKHVDAIK EALSLLNDMR ALENEKNEDV DIISNEFSIQ RPTCVQTRLK LYKQGLRGNL TKLNGALTMI ASHYQTNCPP TPETDCEIEV TTFEDFIKNL KGFLFDIPFD CWKPVQK

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of GM-CSF as determined by LAL method.

Predicted Molecular Mass

14.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

GM-CSF Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine FDC-P1 is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 x 10^8 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat GM-CSF Protein

SDS-PAGE: Recombinant Rat GM-CSF Protein [NBP2-35206]

SDS-PAGE: Recombinant Rat GM-CSF Protein [NBP2-35206]

SDS-Page: Rat GM-CSF Protein [NBP2-35206]

Formulation, Preparation and Storage

NBP2-35206
Formulation Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: GM-CSF

Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is secreted by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimulation. It was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors and has functions of stimulates the growth and differentiation of hematopoietic precursor cells from various lineages. GM-CSF has also been reported to have a functional role on non-hematopoietic cells and can induce human endothelial cells to migrate and proliferate. Additionally, it can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. It is reported that GM-CSF has no biological effects across species.

Long Name

Granulocyte Macrophage Growth Factor

Alternate Names

CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim

Gene Symbol

CSF2

UniProt

Additional GM-CSF Products

Product Documents for Recombinant Rat GM-CSF Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Rat GM-CSF Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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