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Recombinant Human GPR177/WLS His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22947

Novus Biologicals, part of Bio-Techne
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NBP2-22947

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 101-232 of Human GPR177/WLS

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEMSPWF QFMLFILQLD IAFKLNNQIR ENAEVSMDVS LAYRDDAFAE WTEMAHERVP RKLKCTFTSP KTPEHEGRYY ECDVLPFMEI GSVAHKFYLL NIRLPVNEKK KINVGIGEIK DIRLVGIHQN GGFTK

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

17.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GPR177/WLS His Protein

SDS-PAGE: Recombinant Human GPR177/WLS His Protein [NBP2-22947]

SDS-PAGE: Recombinant Human GPR177/WLS His Protein [NBP2-22947]

SDS-Page: Recombinant Human GPR177/WLS Protein [NBP2-22947]

Formulation, Preparation and Storage

NBP2-22947
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GPR177/WLS

WLS, a Wnt trafficking regulator, is a multipass transmembrane protein that regulates the sorting and secretion of Wnt protein. It is required for organogenesis during embryogenesis and for the establishment of the anterior-posterior axis. WLS promotes various cancers such as glioma tumourigenesis. The gene encoding WLS maps to human chromosome 1 and is expressed as multiple alternatively spliced isoforms. Recombinant human WLS protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

C1orf139, Evi, Sprinter, WLS, Wntless

Gene Symbol

WLS

Additional GPR177/WLS Products

Product Documents for Recombinant Human GPR177/WLS His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human GPR177/WLS His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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