Recombinant Human Grancalcin His Protein

Name: Recombinant Human Grancalcin His Protein
Brand: Novus Biologicals
SKU: NBP1-50967

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50967

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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Grancalcin Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-217 of Human Grancalcin

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAYPGYGGGF GNFSIQVPGM QMGQPVPETG PAILLDGYSG PAYSDTYSSA GDSVYTYFSA VAGQDGEVDA EELQRCLTQS GINGTYSPFS LETCRIMIAM LDRDHTGKMG FNAFKELWAA LNAWKENFMT VDQDGSGTVE HHELRQAIGL MGYRLSPQTL TTIVKRYSKN GRIFFDDYVA CCVKLRALTD FFRKRDHLQQ GSANFIYDDF LQGTMAI

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

26.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Grancalcin His Protein

SDS-PAGE: Recombinant Human Grancalcin His Protein [NBP1-50967]

SDS-Page: Recombinant Human Grancalcin His Protein [NBP1-50967] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-50967

Formulation	20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Grancalcin

GCA, also known as Grancalcin, is calcium-binding protein that is especially abundant in human neutrophils. It belongs to the penta EF-hand (PEF) subfamily of EF-hand proteins, which also comprises calpain, sorcin, peflin, and ALG-2. This protein undergoes important conformational changes upon binding of calcium, which subsequently exposes hydrophobic amino acid residues, which direct the protein to hydrophobic surfaces. It interacts with L-plastin, a protein known to have actin bundling activity, which suggests that GCA may play a role in the regulation of neutrophils adhesion. Recombinant human GCA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

EF-hand calcium-binding protein, grancalcin, EF-hand calcium binding protein, grancalcin, penta-EF-hand protein

Gene Symbol

GCA

Additional Grancalcin Products

Product Documents for Recombinant Human Grancalcin His Protein

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COA

Product Specific Notices for Recombinant Human Grancalcin His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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