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Name: Recombinant Human Growth Hormone Protein
Brand: Novus Biologicals
SKU: NBP2-34890

We guarantee this product for 2 years from the date of receipt.

Recombinant Human Growth Hormone Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34890

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P01241

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Growth Hormone Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 191 amino acids corresponding to Growth Hormone Source: E. coli

Uniprot ID: P01241

Amino Acid Sequence: FPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ KYSFLQNPQT SLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV QFLRSVFANS LVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFK QTYSKFDTNS HNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG F

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Growth Hormone as determined by LAL method.

Predicted Molecular Mass

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Growth Hormone protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 lymphoma cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 10^7 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Growth Hormone Protein

SDS-PAGE: Recombinant Human Growth Hormone Protein [NBP2-34890]

SDS-Page: Human Growth Hormone Protein [NBP2-34890]

Formulation, Preparation and Storage

NBP2-34890

Formulation	Lyophilized from a 0.2 um filtered concentrated solution with 20mM PB, pH 7.0.
Preservative	3% Mannitol
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Growth Hormone

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. It is a peptide hormone that stimulates growth, cell reproduction and regeneration in humans and other animals. Growth hormone is a 191-amino acid, single-chain polypeptide that is synthesized, stored, and secreted by somatotropic cells within the lateral wings of the anterior pituitary gland. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones-GH-releasing hormone (GHRH) and somatostatin (SST) as well as by signals from the periphery-ghrelin and leptin. The human GH cDNA encodes a 217 amino acid (a.a.) residue precursor protein with a 26 a.a. putative signal peptide. By alternative splicing, at least four isoforms of GH have been identified.

Alternate Names

GH-N, GH1, GHB5, GHN, IGHD1A, IGHD2, Somatotropin

Gene Symbol

GH1

UniProt

P01241 (Human)

Additional Growth Hormone Products

Product Documents for Recombinant Human Growth Hormone Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human Growth Hormone Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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