Recombinant Human GRP78/HSPA5 His Protein

Name: Recombinant Human GRP78/HSPA5 His Protein
Brand: Novus Biologicals
SKU: NBP3-18319

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18319

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Tag

His

Conjugate

Unconjugated

Applications

Bioactivity, ELISA, Functional Assay, SDS-PAGE, Western Blot

View all GRP78/HSPA5 Proteins and Enzymes »

Product Specifications

Description

A biologically active protein corresponding to human GRP78/HSPA5.

Source: E. coli

Amino Acid Sequence: MKLSLVAAMLLLLSAARAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL

Purity

>85%

Predicted Molecular Mass

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

The protein has ATPase activity at the time of manufacture of 2.3uM phosphate liberated/hr/ug protein in a 200ul reaction at 37C (pH 8) in the presence of 20ul of 1mM ATP using a Malachite Green assay.

Localization

Endoplasmic Reticulum Lumen|Melanosome

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GRP78/HSPA5 His Protein

SDS-PAGE: Recombinant Human GRP78/HSPA5 His Protein [NBP3-18319]

SDS-Page: Recombinant Human GRP78/HSPA5 His Protein [NBP3-18319] - SDS-PAGE of 78kDa Grp78 (Bip) protein.

Formulation, Preparation and Storage

NBP3-18319

Preparation Method	This protein is affinity purified.
Formulation	20mM Tris/HCl pH7.5, 50mM KCl, 5mM MgCl2, 1mM DTT
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20C. Avoid freeze-thaw cycles.

Background: GRP78/HSPA5

Within the ER, misfolded proteins are detected by Bip (GPR78). In the presence of misfolded proteins, GPR78 disscociates from PERK, allowing it to homodimerize and autophosphoyate. In its dimerized phosphorylated form, PERK phosphorylates eIF2. The phosphorylation of eIF2 blocks the majority of translation to prevent the continued accumulation of protein in the ER. GRP78 also binds to IRE1 and ATF6 under normal ER conditions, but dissociates upon accumulation of misfolded proteins. Unbound ATF6 is translocated to the gogli where it is converted into a cleaved, active form. The cleaved form of ATF6 is then able to up regulate transcription of UPR genes including XBP1. Activated IRE1 acts as an endoribonuclease to an intron from XBP1 transcripts. The XBP1 splice variant codes for an active transcription factor which activates transcription of P58IPK. P58IPK is a HSP40 protein which binds to and inhibits PERK. Thus, if the accumulated misfolded proteins have been removed, P58IPK acts to shut down the UPR by removing the block to translation.

Long Name

75 KD Glucose Regulated Protein

Alternate Names

BIP, HSP70-5, HSPA5

Gene Symbol

HSPA5

Additional GRP78/HSPA5 Products

Product Documents for Recombinant Human GRP78/HSPA5 His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human GRP78/HSPA5 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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