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Recombinant Human GSTA1 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30307

Novus Biologicals, part of Bio-Techne
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NBP1-30307

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 1-222 of Human GSTA1

Source: E.coli

Amino Acid Sequence: MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

25.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 35,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GSTA1 Protein

SDS-PAGE: Recombinant Human GSTA1 Protein [NBP1-30307]

SDS-PAGE: Recombinant Human GSTA1 Protein [NBP1-30307]

SDS-Page: GSTA1 Protein [NBP1-30307] - GSTA1, 25.6 kDa (222aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Formulation, Preparation and Storage

NBP1-30307
Formulation 20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GSTA1

GSTA1, also known as Glutathione S-transferase alpha 1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTA1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Glutathione S-transferase A1

Alternate Names

EC 1.11.1.-, EC 2.5.1.18, EC 5.3.3.-, GST HA subunit 1, GST-epsilon, GSTA1-1

Gene Symbol

GSTA1

Additional GSTA1 Products

Product Documents for Recombinant Human GSTA1 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human GSTA1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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