Recombinant Human HARS His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48599

Novus Biologicals, part of Bio-Techne
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NBP1-48599-0.25mg
NBP1-48599-0.05mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all HARS Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-509 of Human HARS

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAERAAL EELVKLQGER VRGLKQQKAS AELIEEEVAK LLKLKAQLGP DESKQKFVLK TPKGTRDYSP RQMAVREKVF DVIIRCFKRH GAEVIDTPVF ELKETLMGKY GEDSKLIYDL KDQGGELLSL RYDLTVPFAR YLAMNKLTNI KRYHIAKVYR RDNPAMTRGR YREFYQCDFD IAGNFDPMIP DAECLKIMCE ILSSLQIGDF LVKVNDRRIL DGMFAICGVS DSKFRTICSS VDKLDKVSWE EVKNEMVGEK GLAPEVADRI GDYVQQHGGV SLVEQLLQDP KLSQNKQALE GLGDLKLLFE YLTLFGIDDK ISFDLSLARG LDYYTGVIYE AVLLQTPAQA GEEPLGVGSV AAGGRYDGLV GMFDPKGRKV PCVGLSIGVE RIFSIVEQRL EALEEKIRTT ETQVLVASAQ KKLLEERLKL VSELWDAGIK AELLYKKNPK LLNQLQYCEE AGIPLVAIIG EQELKDGVIK LRSVTSREEV DVRREDLVEE IKRRTGQPLC IC

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

59.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human HARS His Protein

SDS-PAGE: Recombinant Human HARS His Protein [NBP1-48599]

SDS-PAGE: Recombinant Human HARS His Protein [NBP1-48599]

SDS-Page: Recombinant Human HARS His Protein [NBP1-48599] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-48599
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT, 0.1 M NaCl
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HARS

HARS, also known as Histidyl-tRNA synthetase, function to catalyze the aminoacylation of tRNAs by their corresponding amino acids. HARS is from the class II family of aminoacyl-tRNA synthetases. It is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Recombinant human HARS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Alternate Names

cytoplasmic, EC 6.1.1.21, FLJ20491, histidine-tRNA ligase, histidyl-tRNA synthetase, histidyl-tRNA synthetase, cytoplasmic

Gene Symbol

HARS1

Additional HARS Products

Product Documents for Recombinant Human HARS His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human HARS His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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