Recombinant Human HB-EGF Protein

Name: Recombinant Human HB-EGF Protein
Brand: Novus Biologicals
SKU: NBP2-34920

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34920

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Product Specifications
Scientific Data
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Background
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Key Product Details

Source

E. coli

Accession #

Q99075

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all HB-EGF Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 86 amino acids corresponding to HB-EGF Source: E. coli

Uniprot ID: Q99075

Amino Acid Sequence: DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of HB-EGF as determined by LAL method.

Predicted Molecular Mass

9.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

HB-EGF protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human HB-EGF Protein

SDS-PAGE: Recombinant Human HB-EGF Protein [NBP2-34920]

SDS-Page: Human HB-EGF Protein [NBP2-34920]

Formulation, Preparation and Storage

NBP2-34920

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 10 mM PBS, 500 mM NaCl, pH 7.4.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.3 mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: HB-EGF

Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation, wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Human HB-EGF shares about 73 % and 76 % a.a. sequence identity with murine and rat HB-EGF.

Long Name

Heparin Binding EGF-like Growth Factor

Alternate Names

Dtr, Dts, HBEGF, Hegfl

Gene Symbol

HBEGF

UniProt