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Recombinant Human HES2 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23099

Novus Biologicals, part of Bio-Techne
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NBP2-23099

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-173 of Human HES2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGLPRRA GDAAELRKSL KPLLEKRRRA RINQSLSQLK GLILPLLGRE NSNCSKLEKA DVLEMTVRFL QELPASSWPT AAPLPCDSYR EGYSACVARL ARVLPACRVL EPAVSARLLE HLWRRAASAT LDGGRAGDSS GPSAPAPAPA SAPEPASAPV PSPPSPPCGP GLWRPW

Purity

>80%, by SDS-PAGE

Predicted Molecular Mass

20.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human HES2 His Protein

SDS-PAGE: Recombinant Human HES2 His Protein [NBP2-23099]

SDS-PAGE: Recombinant Human HES2 His Protein [NBP2-23099]

SDS-Page: HES2 Protein [NBP2-23099]

Formulation, Preparation and Storage

NBP2-23099
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HES2

Hairy and enhancer of split 2, also known as HES2, is a member of the HES family and contains one basic helix-loop-helix (bHLH) domain and one orange domain. The HES family members form a complex with TLE, the mammalian homologue of Groucho, and this interaction is mediated by the carboxy terminal WRPW motif of the HES proteins. Activation of Notch signaling pathway leads to activation of HES family genes through the interaction between Notch intracellular domain and RBPSUH (CSL). HES2 is expressed in a variety of adult and embryonic tissue. Recombinant human HES2 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

BHLHB40, bHLHb40Class B basic helix-loop-helix protein 40, Hairy and enhancer of split 2, hairy and enhancer of split 2 (Drosophila), transcription factor HES-2

Gene Symbol

HES2

Additional HES2 Products

Product Documents for Recombinant Human HES2 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human HES2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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