Amino Acid Sequence: MADEIAKAQV ARPGGDTIFG KIIRKEIPAK IIFEDDRCLA FHDISPQAPT HFLVIPKKHI SQISVAEDDD ESLLGHLMIV GKKCAADLGL NKGYRMVVNE GSDGGQSVYH VHLHVLGGRQ MHWPPG

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

13.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Use in Microarray reported in scientific literature (PMID:35059422)

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human HINT1 Protein

Recombinant Human HINT1 Protein [NBC1-28612]

Formulation, Preparation and Storage

NBC1-28612

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HINT1

HINT1, also known as Histidine triad nucleotide-binding protein 1, is a member of superfamily named for a near C-terminal HXHXHXX motif (H:Histidine, X:a hydrophobic amino acid) positioned at the alpha-phosphate of nucleotide substrates. This protein hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2. Although it was originally thought to be a protein kinase C inhibitor and act as a haplod-insufficient tumor suppressor including spontaneous tumor formation in Hint+/- and Hint-/- , its actual physiologic function is not known. Recombinant human HINT1 protein was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

Adenosine 5'-monophosphoramidase, EC 3.-, HINT, histidine triad nucleotide binding protein 1, histidine triad nucleotide-binding protein, histidine triad nucleotide-binding protein 1, PKCI1, PKCI-1FLJ30414, PRKCNH1FLJ32340, Protein kinase C inhibitor 1, Protein kinase C-interacting protein 1

Gene Symbol

HINT1

Additional HINT1 Products

Product Documents for Recombinant Human HINT1 Protein

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for Recombinant Human HINT1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations
Reviews

Recombinant Human HINT1 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-28612

Key Product Details

Source

Conjugate

Applications

View all HINT1 Proteins and Enzymes »

Product Specifications

Description