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Name: Recombinant Human Histone Deacetylase 2/HDAC2 His Protein
Brand: Novus Biologicals
SKU: NBP1-72399

NBP1-72399 was developed and has only been validated for use in SDS-PAGE. We have not evaluated the functional activity of this protein, and it's possible that it may not possess biological activity at all and we cannot guarantee it. It is suitable for use as a positive control in western blotting and potentially ELISA.

Recombinant Human Histone Deacetylase 2/HDAC2 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72399

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Baculovirus

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Histone Deacetylase 2/HDAC2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-488 of Human Histone Deacetylase 2/HDAC2

Source: Baculovirus

Amino Acid Sequence: MAYSQGGGKK KVCYYYDGDI GNYYYGQGHP MKPHRIRMTH NLLLNYGLYR KMEIYRPHKA TAEEMTKYHS DEYIKFLRSI RPDNMSEYSK QMQRFNVGED CPVFDGLFEF CQLSTGGSVA GAVKLNRQQT DMAVNWAGGL HHAKKSEASG FCYVNDIVLA ILELLKYHQR VLYIDIDIHH GDGVEEAFYT TDRVMTVSFH KYGEYFPGTG DLRDIGAGKG KYYAVNFPMR DGIDDESYGQ IFKPIISKVM EMYQPSAVVL QCGADSLSGD RLGCFNLTVK GHAKCVEVVK TFNLPLLMLG GGGYTIRNVA RCWTYETAVA LDCEIPNELP YNDYFEYFGP DFKLHISPSN MTNQNTPEYM EKIKQRLFEN LRMLPHAPGV QMQAIPEDAV HEDSGDEDGE DPDKRISIRA SDKRIACDEE FSDSEDEGEG GRRNVADHKK GAKKARIEED KKETEDKKTD VKEEDKSKDN SGEKTDTKGT KSEQLSNPSR HHHHHH

Purity

>85%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

56.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Histone Deacetylase 2/HDAC2 His Protein

SDS-PAGE: Recombinant Human Histone Deacetylase 2/HDAC2 His Protein [NBP1-72399]

SDS-Page: Recombinant Human Histone Deacetylase 2/HDAC2 Protein [NBP1-72399] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-72399

Formulation	20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT, 0.1 mM PMSF
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 2/HDAC2

HDAC2 belongs to the histone deacetylase family that act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones. It forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. It also plays an important role in transcriptional regulation, cell cycle progression and developmental events. Recombinant human HDAC2 protein was expressed with c-terminal His-tag in high-5 cells using baculovirus expression system and purified by using conventional chromatography techniques.

Long Name

Histone Deacetylase 2

Alternate Names

HDAC2, YAF1

Gene Symbol

HDAC2

Additional Histone Deacetylase 2/HDAC2 Products

Product Documents for Recombinant Human Histone Deacetylase 2/HDAC2 His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human Histone Deacetylase 2/HDAC2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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