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Recombinant Human Histone H3.3A His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23098

Novus Biologicals, part of Bio-Techne
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NBP2-23098

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-136 of Human Histone H3.3A

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMARTKQT ARKSTGGKAP RKQLATKAAR KSAPSTGGVK KPHRYRPGTV ALREIRRYQK STELLIRKLP FQRLVREIAQ DFKTDLRFQS AAIGALQEAS EAYLVGLFED TNLCAIHAKR VTIMPKDIQL ARRIRGERA

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

17.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Histone H3.3A His Protein

SDS-PAGE: Recombinant Human Histone H3.3A His Protein [NBP2-23098]

SDS-PAGE: Recombinant Human Histone H3.3A His Protein [NBP2-23098]

SDS-Page: Histone H3.3A Protein [NBP2-23098]

Formulation, Preparation and Storage

NBP2-23098
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H3.3A

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H3F3A is a replication-independent member of the histone H3 family. Recombinant human H3F3A protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

H3 histone, family 3A, H3.3AH3F3H3F3B, H3.3B, histone H3.3, MGC87782, MGC87783

Gene Symbol

H3-3A

Additional Histone H3.3A Products

Product Documents for Recombinant Human Histone H3.3A His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human Histone H3.3A His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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