Recombinant Human HMGCL His Protein

Name: Recombinant Human HMGCL His Protein
Brand: Novus Biologicals
SKU: NBP1-78844

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78844

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all HMGCL Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 28-325 of Human HMGCL

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMTLPKR VKIVEVGPRD GLQNEKNIVS TPVKIKLIDM LSEAGLSVIE TTSFVSPKWV PQMGDHTEVL KGIQKFPGIN YPVLTPNLKG FEAAVAAGAK EVVIFGAASE LFTKKNINCS IEESFQRFDA ILKAAQSANI SVRGYVSCAL GCPYEGKISP AKVAEVTKKF YSMGCYEISL GDTIGVGTPG IMKDMLSAVM QEVPLAALAV HCHDTYGQAL ANTLMALQMG VSVVDSSVAG LGGCPYAQGA SGNLATEDLV YMLEGLGIHT GVNLQKLLEA GNFICQALNR KTSSKVAQAT CKL

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

34.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human HMGCL His Protein

SDS-PAGE: Recombinant Human HMGCL His Protein [NBP1-78844]

SDS-Page: Recombinant Human HMGCL Protein [NBP1-78844] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-78844

Formulation	20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGCL

HMGCL, also known as hydroxymethylglutaryl-CoA lyase, is a mitochondrial matrix protein that belongs to the HMG-CoA lyase family. It exists as a homodimer and participates in leucine catabolism and ketogenesis, the hepatic synthesis of ketone bodies that, during fasting, provide a major source of energy for heart, brain and kidney. More specifically, it catalyzes the final step of these processes, the cleavage of 3-hydroxy-3-methylglutaryl-CoA to acetoacetic acid and acetyl-CoA. Recombinant human HMGCL protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

3-hydroxy-3-methylglutaryl-CoA lyase, 3-hydroxymethyl-3-methylglutaryl-CoA lyase, EC 4.1.3.4, HLmitochondrial

Gene Symbol

HMGCL

Additional HMGCL Products

Product Documents for Recombinant Human HMGCL His Protein

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COA

Product Specific Notices for Recombinant Human HMGCL His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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