Horse IgG, Fc Native Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97034
![SDS-PAGE: Horse IgG, Fc Native Protein [NBP1-97034]](https://resources.bio-techne.com/images/products/Horse-IgG--Fc-Native-Protein-SDS-Page-NBP1-97034-img0007.jpg "SDS-PAGE: Horse IgG, Fc Native Protein [NBP1-97034]")
Conjugate
Catalog #
Key Product Details
Conjugate
Unconjugated
Applications
ELISA, SDS-PAGE, Western Blot
Product Specifications
Description
Native Equine Horse IgG, Fc
Source: Normal serum
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis.
Source: Normal serum
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis.
Purity
Multi-step
Application Notes
This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Suggested application: IHC.
Specificity
Assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse Serum, anti-Horse IgG and anti-Horse IgG F(c). No reaction was observed against anti-Horse IgG F(ab')2 or anti-Papain.
Protein / Peptide Type
Native Protein
Scientific Data Images for Horse IgG, Fc Native Protein
SDS-PAGE: Horse IgG, Fc Native Protein [NBP1-97034]
SDS-Page: Horse IgG, Fc Native Protein [NBP1-97034] - Lane 1: Horse IgG Fragment Reduced. Lane 2: Horse IgG Fragment Non reduced. Load: 1.0ug per lane.Formulation, Preparation and Storage
NBP1-97034
| Preparation Method | This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Horse IgG F(c) fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse Serum, anti-Horse IgG and anti-Horse IgG F(c). No reaction was observed against anti-Horse IgG F(ab')2 or anti-Papain. |
| Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Concentration | 1 mg/ml |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: Horse IgG, Fc
Additional Horse IgG, Fc Products
Product Documents for Horse IgG, Fc Native Protein
Product Specific Notices for Horse IgG, Fc Native Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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