Recombinant E. coli HSP70/HSPA1A Protein

Name: Recombinant E. coli HSP70/HSPA1A Protein
Brand: Novus Biologicals
SKU: NBC1-18356

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-18356

Submit a review

Product Datasheet / COA

100% Guarantee

Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

SDS-PAGE

View all HSP70/HSPA1A Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 385-546 of E.coli HSP70/HSPA1A

Source: E.coli

Amino Acid Sequence: MDVKDVLLLD VTPLSLGIET MGGVMTTLIA KNTTIPTKHS QVFSTAEDNQ SAVTIHVLQG ERKRAADNKS LGQFNLDGIN PAPRGMPQIE VTFDIDADGI LHVSAKDKNS GKEQKITIKA SSGLNEDEIQ KMVRDAEANA EADRKFEELV QTRNQGDHLL HST

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

17.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli HSP70/HSPA1A Protein

SDS-PAGE: Recombinant E. coli HSP70/HSPA1A Protein [NBC1-18356]

SDS-Page: Hsp70 Protein [NBC1-18356] - Dna K, 17.7kDa (163aa) with a purity of 95% by SDS - PAGE

Formulation, Preparation and Storage

NBC1-18356

Formulation	25 mM Tris-HCl buffer (pH7.5), 2 mM beta-mercaptoethanol, 1 mM EDTA
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP70/HSPA1A

DNAK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid(Met) is attached at N- terminus

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Gene Symbol

HSPA1A

Additional HSP70/HSPA1A Products

Product Documents for Recombinant E. coli HSP70/HSPA1A Protein

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for Recombinant E. coli HSP70/HSPA1A Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations
Reviews