Human alpha 2-Macroglobulin Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1938-PI
Key Product Details
Source
Human Plasma
Structure / Form
Disulfide-linked homo-oligomer
Conjugate
Unconjugated
Applications
Inhibition Activity
Product Specifications
Source
Human plasma-derived alpha 2-Macroglobulin protein
The human plasma used for the isolation of this product was certified by the supplier to be HIV-1 and HBsAg negative at the time of shipment. Human blood products should always be treated in accordance with universal handling precautions.
The human plasma used for the isolation of this product was certified by the supplier to be HIV-1 and HBsAg negative at the time of shipment. Human blood products should always be treated in accordance with universal handling precautions.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser24
SDS-PAGE
90-170 kDa, reducing conditions
Activity
Measured by its ability to trap trypsin. The trapped trypsin is no longer able to interact with protein substrates or inhibitors, but still able to cleave small peptide substrates or inhibitors.
Human alpha2-Macroglobulin has an IC50 value of <5 nM, as measured under the described conditions.
Human alpha2-Macroglobulin has an IC50 value of <5 nM, as measured under the described conditions.
Formulation, Preparation and Storage
1938-PI
| Formulation | Supplied as a 0.2 μm filtered solution in NaH2PO4, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: alpha 2-Macroglobulin
Human alpha2-macroglobulin (h alpha2M) is a serum glycoprotein that has sequence similarity to other members of the alpha2M family including complement components C3, C4 and C5 (1). alpha2M is synthesized as a polypeptide of 1474 amino acids with a signal peptide (23 residues) (2). The mature protein is a tetramer (720 kDa) of 4 identical subunits (180 kDa), which form two disulfide bond-linked dimers. As a general and irreversible protease inhibitor implicated in many processes, alpha2M is able to inhibit all four classes of proteases by a unique trapping mechanism. The bait region of h alpha2M (residues 690‑728) contains specific cleavage sites for different proteases. The cleavage of the bait region by a protease induces a conformation change in alpha2M, which then traps and forms a covalent bond with the protease. The trapped protease remains active against small peptide substrates but loses its ability to interact with large protein substrates or inhibitors.
References
- Sottrup-Jensen, L. et al. (1985) Proc. Natl. Acad. Sci. USA 82:9.
- Kan, C.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82:2282.
Alternate Names
A2M, alpha 2Macroglobulin, CPAMD5
Entrez Gene IDs
2 (Human)
Gene Symbol
A2M
Additional alpha 2-Macroglobulin Products
Product Documents for Human alpha 2-Macroglobulin Protein, CF
Product Specific Notices for Human alpha 2-Macroglobulin Protein, CF
For research use only
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