Human Serpin G1/C1 Inhibitor (Plasma) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2488-PI
Key Product Details
Source
Human Plasma
Conjugate
Unconjugated
Applications
Inhibition Activity
Product Specifications
Source
Human plasma-derived Serpin G1/C1 Inhibitor protein
The human plasma used for the isolation of this product were certified by the supplier to be HIV-1 and HBsAg negative at the time of shipment. Human blood products should always be treated in accordance with universal handling precautions.
The human plasma used for the isolation of this product were certified by the supplier to be HIV-1 and HBsAg negative at the time of shipment. Human blood products should always be treated in accordance with universal handling precautions.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
NPNATSSS
SDS-PAGE
93 kDa, reducing conditions
Activity
Measured by its ability to inhibit Recombinant Human Complement Component C1s (Catalog # 2060-SE) cleavage of a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-K-SBzl).
The IC50 is <2.6 nM, as measured under the described conditions.
The IC50 is <2.6 nM, as measured under the described conditions.
Formulation, Preparation and Storage
2488-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in Sodium Acetate and NaCl. |
| Reconstitution |
Reconstitute at 0.5 mg/mL in sterile, deionized water.
|
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Serpin G1/C1 Inhibitor
As a member of the serpin superfamily of serine protease inhibitors, Serpin G1/C1 inhibitor is the physiological inhibitor of activated C1r and C1s, two serine proteases involved in the classical complement pathway. In addition, it inhibits plasma kallikrein and coagulation factor XIIa, two serine proteases involved in the processing of kininogen to release bradykinin. Therefore, it plays an important role in regulating activation of both the complement and contact systems (1). Serpin G1 deficiency results in hereditary angioedema, which is characterized by recurrent episodes of localized angioedema of the skin, gastrointestinal mucosa or upper respiratory mucosa (2). The deduced amino acid sequence of human Serpin G1 precursor consists of 500 residues with a signal peptide. The mature protein of 478 amino acid residues is heavily glycosylated (1).
References
- Davis, A.E. III et al. (1993) Methods Enzymol. 223:97.
- Davis, A.E. III (2004) Drug News Perspect. 17:439.
Alternate Names
C1 Inhibitor
Entrez Gene IDs
710 (Human)
Gene Symbol
SERPING1
Additional Serpin G1/C1 Inhibitor Products
Product Documents for Human Serpin G1/C1 Inhibitor (Plasma) Protein, CF
Product Specific Notices for Human Serpin G1/C1 Inhibitor (Plasma) Protein, CF
For research use only
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