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Recombinant Human IFN-alpha 2 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61318

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-61318-500ug
NBP2-61318-1mg
NBP2-61318-10ug
NBP2-61318-100ug

Key Product Details

Source

Yeast

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Description

A single polypeptide chain containing 165 amino acids corresponding to IFN-alpha 2 Source: Yeast

Amino Acid Sequence: CDLPQTHSLG SRRTLMLLAQ MRRISLFSCL KDRRDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of IFN-alpha 2 as determined by LAL method.

Predicted Molecular Mass

19.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The activity is determined by the cytopathic effect inhibition assay.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-61318
Formulation Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4, with 0.02 % Tween-20.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IFN-alpha 2/IFNA2

In the human genome, the IFN-omega gene family consists of seven members located on chromosome 9. However, only one of these genes is functional giving rise to the IFN-omega protein, whereas the others are non-functional pseudogenes. The IFN-omega gene is not expressed in unstimulated cells. Viral infection results in expression of the gene giving rise to the N-glycosylated protein consisting of 172 or 174 amino acids and an apparent molecular mass of about 25kDa. A single carbohydrate group consists mainly of biantennary complex oligosaccharides with variable amounts of N-acetyl neuraminic acid. In quantitative terms, IFN-omega is a major component of human leukocyte IFN; with a contribution to its total antiviral activity estimated to be in the range of 10 -15 %.

Long Name

Interferon alpha 2

Alternate Names

Ifa2, IFNA2, IFNA2a, IFNA2b, IFNA2c, IFNAA, IFNalpha 2

Gene Symbol

IFNA2

Additional IFN-alpha 2/IFNA2 Products

Product Documents for Recombinant Human IFN-alpha 2 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human IFN-alpha 2 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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