Recombinant Canine IFN-gamma Protein

Name: Recombinant Canine IFN-gamma Protein
Brand: Novus Biologicals
SKU: NBP2-34894

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34894

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P42161

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IFN-gamma Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 143 amino acids corresponding to IFN-gamma Source: E. coli

Uniprot ID: P42161

Amino Acid Sequence: QAMFFKEIEN LKEYFNASNP DVSDGGSLFV DILKKWREES DKTIIQSQIV SFYLKLFDNF KDNQIIQRSM DTIKEDMLGK FLNSSTSKRE DFLKLIQIPV NDLQVQRKAI NELIKVMNDL SPRSNLRKRK RSQNLFRGRR ASK

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of IFN-gamma as determined by LAL method.

Predicted Molecular Mass

16.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using A-72 canine fibroma cells infected with vesicular stomatitis virus (VSV) is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Canine IFN-gamma Protein

SDS-PAGE: Recombinant Canine IFN-gamma Protein [NBP2-34894]

SDS-Page: Canine IFN-gamma Protein [NBP2-34894]

Formulation, Preparation and Storage

NBP2-34894

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 25 mM Sodium Succinate, pH 5.0, 60 mM NaCl, with 0.1 % Tween-80.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IFN-gamma

Interferon-gamma (IFN-gamma), also known as Type II interferon or immune interferon, is a cytokine produced primarily by Tlymphocytes and natural killer cells. The protein shares no significant homology with IFN-beta or the various IFN-alpha family proteins. Mature IFN-gamma exists as noncovalently-linked homodimers. IFN-gamma was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-gamma induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. Additionally, IFN-gamma augments TH1 cell expansion and may be required for TH1 cell differentiation. Canine IFN-gamma shares 79 %~88 % amino acid sequence identity with bovine, equine and feline IFN-gamma, 62 %~73 % with human, porcine and rhesus macaque IFN-gamma, and 40 %~47 % with cotton rat, murine and rat IFN-gamma.

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Gene Symbol

IFNG

UniProt