Recombinant Human IFN-omega/IFNW1 Protein

Name: Recombinant Human IFN-omega/IFNW1 Protein
Brand: Novus Biologicals
SKU: NBP2-35893

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35893

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IFN-omega Proteins and Enzymes »

Product Specifications

Description

containing 172 amino acid residues with two conserved disulfide bonds corresponding to IFN-omega/IFNW1 Source: E. coli

Uniprot ID: P05000

Amino Acid Sequence: CDLPQNHGLL SRNTLVLLHQ MRRISPFLCL KDRRDFRFPQ EMVKGSQLQK AHVMSVLHEM LQQIFSLFHT ERSSAAWNMT LLDQLHTGLH QQLQHLETCL LQVVGEGESA GAISSPALTL RRYFQGIRVY LKEKKYSDCA WEVVRMEIMK SLFLSTNMQE RLRSKDRDLG SS

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IFN-omega/IFNW1 as determined by LAL method.

Predicted Molecular Mass

20.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human TF-1 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 x 10^8 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IFN-omega/IFNW1 Protein

SDS-PAGE: Recombinant Human IFN-omega/IFNW1 Protein [NBP2-35893]

SDS-Page: Human IFN-omega/IFNW1 Protein [NBP2-35893]

Formulation, Preparation and Storage

NBP2-35893

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IFN-omega

Interferon-Omega (IFN-omega) coded by IFNW1 gene in human, is a number of the type I interferon family, which includes IFN-alpha, IFN-beta, and IFN-omega. The IFNAR-1/IFNAR-2 receptor complex can help with the signal transduction, followed the antiviral or the antiproliferative actions. IFN-omega is derived from IFN-alpha/beta and shares 75 % sequence homology with IFN-alpha. It has two intramolecular disulfide bonds which are crucial for activities. Mire-Sluis et al have described bioassays for IFN-alpha, IFN-beta, and IFN-omega that exploit the ability of these factors to inhibit proliferation of TF-1 cells induced by GM-CSF. The bioassays can be used also with Epo and TF-1 cells, or Epo and Epo-transfected UT-7 cells.

Long Name

Interferon omega

Alternate Names

IFNomega, IFNW1

Gene Symbol

IFNW1

Additional IFN-omega Products

Product Documents for Recombinant Human IFN-omega/IFNW1 Protein

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COA

Product Specific Notices for Recombinant Human IFN-omega/IFNW1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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