Recombinant Human IGF-I/IGF-1 Protein

Name: Recombinant Human IGF-I/IGF-1 Protein
Brand: Novus Biologicals
SKU: NBP2-35000

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35000

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P05019

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IGF-I/IGF-1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 70 amino acids corresponding to IGF-I/IGF-1 Source: E. coli

Uniprot ID: P05019

Amino Acid Sequence: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.01 EU/ug of IGF-I/IGF-1 as determined by LAL method.

Predicted Molecular Mass

7.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IGF1 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IGF-I/IGF-1 Protein

SDS-PAGE: Recombinant Human IGF-I/IGF-1 Protein [NBP2-35000]

SDS-Page: Human IGF-I Protein [NBP2-35000]

Formulation, Preparation and Storage

NBP2-35000

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IGF-I/IGF-1

Insulin-like Growth Factor I (IGF-I), also known as Somatomedin C, is the dominant effector of Growth Hormone (GH). IGF-I regulates glucose, fatty acid, and protein metabolism, steroid hormone activity, cartilage and bone metabolism, muscle regeneration, tumor progression, and cellular differentiation and migration. IGF-I effects are mediated primarily by IGF-I R, and its bioavailability is regulated by IGF binding proteins (IGFBPs). Mature human IGF-I shares about 95% aa sequence identity with the mouse and rat IGF-I. Long R3 IGF-I (LR3 IGF-I) is a synthetic analog of IGF-I that has a Glu3Arg substitution and a 13 aa N-terminal extension derived from methionyl porcine GH. It retains the ability to bind IGF-I R and Insulin R but shows reduced affinity for IGFBPs. As a result, LR3 IGF-I has an increased half-life and displays increased biological potency compared to IGF-I.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Gene Symbol

IGF1

UniProt