Recombinant Mouse IGF-I/IGF-1 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35081

Novus Biologicals, part of Bio-Techne
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Key Product Details

Source

E. coli

Accession #

P05017

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IGF-I/IGF-1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 70 amino acids corresponding to IGF-I/IGF-1 Source: E. coli

Uniprot ID: P05017

Amino Acid Sequence: GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of IGF-I/IGF-1 as determined by LAL method.

Predicted Molecular Mass

7.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IGF-I/IGF-1 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse IGF-I/IGF-1 Protein

SDS-PAGE: Recombinant Mouse IGF-I/IGF-1 Protein [NBP2-35081]

SDS-PAGE: Recombinant Mouse IGF-I/IGF-1 Protein [NBP2-35081]

SDS-Page: Mouse IGF-I Protein [NBP2-35081]

Formulation, Preparation and Storage

NBP2-35081
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in 100mM HAc to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IGF-I/IGF-1

Insulin-like Growth Factor I (IGF-I), also known as Somatomedin C, is the dominant effector of Growth Hormone (GH). IGF-I regulates glucose, fatty acid, and protein metabolism, steroid hormone activity, cartilage and bone metabolism, muscle regeneration, tumor progression, and cellular differentiation and migration. IGF-I effects are mediated primarily by IGF-I R, and its bioavailability is regulated by IGF binding proteins (IGFBPs). Mature human IGF-I shares about 95% aa sequence identity with the mouse and rat IGF-I. Long R3 IGF-I (LR3 IGF-I) is a synthetic analog of IGF-I that has a Glu3Arg substitution and a 13 aa N-terminal extension derived from methionyl porcine GH. It retains the ability to bind IGF-I R and Insulin R but shows reduced affinity for IGFBPs. As a result, LR3 IGF-I has an increased half-life and displays increased biological potency compared to IGF-I.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Gene Symbol

IGF1

UniProt

P05017

Additional IGF-I/IGF-1 Products

Product Documents for Recombinant Mouse IGF-I/IGF-1 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Mouse IGF-I/IGF-1 Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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