Recombinant Human IGF-I/IGF-1 15N Stable Isotope Protein

Name: Recombinant Human IGF-I/IGF-1 15N Stable Isotope Protein
Brand: Novus Biologicals
SKU: NBP2-35896

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35896

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P05019

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IGF-I/IGF-1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 70 amino acids corresponding to 15N stable isotope labeled IGF-I/IGF-1 Source: E. coli

Uniprot ID: P05019

Amino Acid Sequence: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IGF-I/IGF-1 as determined by LAL method.

Predicted Molecular Mass

7.74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IGF-I/IGF-1 15N Stable Isotope Protein

SDS-PAGE: Recombinant Human IGF-I/IGF-1 15N Stable Isotope Protein [NBP2-35896]

SDS-Page: Human IGF-I 15N Protein [NBP2-35896]

Formulation, Preparation and Storage

NBP2-35896

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.2.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IGF-I/IGF-1

The insulin-like growth factors (IGFs) belonged to the insulin gene family, are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Recombinant human IGF-1 are globular proteins containing 70 amino acids and 3 intra-molecular disulfide bonds. Mature human IGF-1 shares 94 % and 96 % a.a. sequence identity with mouse and rat IGF-1, respectively, and exhibits cross-species activity.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Gene Symbol

IGF1

UniProt