Recombinant Human IGF-I/IGF-1 LR3 Protein

Name: Recombinant Human IGF-I/IGF-1 LR3 Protein
Brand: Novus Biologicals
SKU: NBP2-61314

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61314

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IGF-I/IGF-1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 83 amino acids corresponding to IGF-I/IGF-1 Source: E. coli

Amino Acid Sequence: MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

Purity

>98%, by SDS-PAGE

Endotoxin Level

Less than 0.01 EU/ug of IGF-I/IGF-1 as determined by LAL method.

Predicted Molecular Mass

9.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Assay 1: Fully biologically active when compared to standard. Measured in a serum-free cell proliferation assay using human MCF-7 cells. The ED50 for this effect is 0.3-1.5 ng/ml, corresponding to a specific activity of > 6.7 x 10^5 U/mg. Assay 2: Fully biologically active when compared to standard. The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10 ng/ml, corresponding to a specific activity of > 1.0 x 10^5 U/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IGF-I/IGF-1 LR3 Protein

SDS-Page: Recombinant Human IGF-I/IGF-1 LR3 Protein [NBP2-61314]

Formulation, Preparation and Storage

NBP2-61314

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.2.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IGF-I/IGF-1

IGF-1 belonged to the insulin gene family, is a mitogenic polypeptide growth factor that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. It is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. The LR3IGF-1 is a long-term analog of human IGF-1, specifically designed and manufactured for mammalian cell culture to support large-scale manufacturing of recombinant biopharmaceuticals.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Gene Symbol

IGF1

Additional IGF-I/IGF-1 Products

Product Documents for Recombinant Human IGF-I/IGF-1 LR3 Protein

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COA

Product Specific Notices for Recombinant Human IGF-I/IGF-1 LR3 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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