Recombinant Human IGFBP-5 Protein

Name: Recombinant Human IGFBP-5 Protein
Brand: Novus Biologicals
SKU: NBP2-61313

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61313

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Product Specifications
Scientific Data
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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

View all IGFBP-5 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 252 amino acids corresponding to IGFBP-5 Source: E. coli

Uniprot ID: P24593

Amino Acid Sequence: LGSFVHCEPC DEKALSMCPP SPLGCELVKE PGCGCCMTCA LAEGQSCGVY TERCAQGLRC LPRQDEEKPL HALLHGRGVC LNEKSYREQV KIERDSREHE EPTTSEMAEE TYSPKIFRPK HTRISELKAE AVKKDRRKKL TQSKFVGGAE NTAHPRIISA PEMRQESEQG PCRRHMEASL QELKASPRMV PRAVYLPNCD RKGFYKRKQC KPSRGRKRGI CWCVDKYGMK LPGMEYVDGD FQCHTFDSSN VE

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of IGFBP-5 as determined by LAL method.

Predicted Molecular Mass

28.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.4 ug/ml, corresponding to a specific activity of > 2500 IU/mg in the presence of 15 ng/ml of rHuIGF-II.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IGFBP-5 Protein

SDS-Page: Recombinant Human IGFBP-5 Protein [NBP2-61313]

Formulation, Preparation and Storage

NBP2-61313

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 10 mM Sodium Citrate, pH 3.0.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IGFBP-5

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors

Long Name

Insulin-like Growth Factor Binding Protein 5

Alternate Names

IGFBP5

Gene Symbol