Recombinant Human IgL GST (N-Term) Protein

Name: Recombinant Human IgL GST (N-Term) Protein
Brand: Novus Biologicals
SKU: H00003535-P01

Novus Biologicals, part of Bio-Techne | Catalog # H00003535-P01

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Wheat germ

Tag

GST (N-Term)

Conjugate

Unconjugated

Applications

ELISA, Affinity Purification, Microarray, SDS-PAGE, Western Blot

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Product Specifications

Description

Recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-232 of Human IGL@

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MAWTPLLLPLLTFCTVSEASYDLTQPPSVSVSPGQTARITCSGDALPRKYAFWYQQKSGQAPVLVIYEDSKRPSGIPERFSGSSSGTMATLTISGAQVEDEGDYYCYSTDISGYPVFGGGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWRSHKSYSCQVTHEGSTVEKTVAPTECS

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

51.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IgL GST (N-Term) Protein

SDS-PAGE: Recombinant Human IgL GST (N-Term) Protein [H00003535-P01]

SDS-Page: Recombinant Human IgL GST (N-Term) Protein [H00003535-P01] - Recombinant Human IgL Protein [H00003535-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Formulation, Preparation and Storage

H00003535-P01

Preparation Method	in vitro wheat germ expression system
Formulation	50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: IgL

Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the lambda light chain locus. The locus includes V (variable), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Several V segments and three C segments are known to be incapable of encoding a protein and are considered pseudogenes. The locus also includes several non-immunoglobulin genes, many of which are pseudogenes or are predicted by automated computational analysis or homology to other species. [provided by RefSeq]

Alternate Names

ig lambda-6 chain C region, IGL, IGLC6, immunoglobulin lambda gene cluster, immunoglobulin lambda locus, Lambda Light Chain, MGC88804

Gene Symbol

IGL

Additional IgL Products

Product Documents for Recombinant Human IgL GST (N-Term) Protein

Product Datasheets

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COA

SDS

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Product Specific Notices for Recombinant Human IgL GST (N-Term) Protein

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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