Recombinant Rhesus Macaque IL-1 alpha/IL-1F1 Protein

Name: Recombinant Rhesus Macaque IL-1 alpha/IL-1F1 Protein
Brand: Novus Biologicals
SKU: NBP2-35305

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35305

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Product Specifications
Scientific Data
Preparation & Storage
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Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-1 alpha/IL-1F1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 159 amino acids corresponding to IL-1 alpha/IL-1F1 Source: E. coli

Uniprot ID: P48089

Amino Acid Sequence: SAPFSFLSNM TYHFIRIIKH EFILNDTLNQ TIIRANDQHL TAAAIHNLDE AVKFDMGAYT SSKDDTKVPV ILRISKTQLY VSAQDEDQPV LLKEMPEINK TITGSETNFL FFWETHGTKN YFISVAHPNL FIATKHDNWV CLAKGLPSIT DFQILENQA

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of IL-1 alpha/IL-1F1 as determined by LAL method.

Predicted Molecular Mass

18.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL1 alpha Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 10 pg/ml, corresponding to a specific activity of > 1.0 x 10^8 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rhesus Macaque IL-1 alpha/IL-1F1 Protein

SDS-PAGE: Recombinant Rhesus Macaque IL-1 alpha/IL-1F1 Protein [NBP2-35305]

SDS-Page: Primate IL-1 alpha/IL-1F1 Protein [NBP2-35305]

Formulation, Preparation and Storage

NBP2-35305

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-1 alpha/IL-1F1

Interleukin-1 alpha (IL-1alpha) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. Recombinant rhesus macaque IL-1 alpha contains 150 amino acids which is a single nonglycosylated polypeptide. Among various species, the amino acid sequence of mature IL-1alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines.

Long Name

Interleukin 1 alpha

Alternate Names

BAF, IL-1A, IL-1F1, IL1, IL1 alpha, IL1A, IL1F1, LAF, LEM

Gene Symbol

IL1A

Additional IL-1 alpha/IL-1F1 Products

Product Documents for Recombinant Rhesus Macaque IL-1 alpha/IL-1F1 Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Rhesus Macaque IL-1 alpha/IL-1F1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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