Recombinant Human IL-17F Protein

Name: Recombinant Human IL-17F Protein
Brand: Novus Biologicals
SKU: NBP2-35046

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35046

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Product Specifications
Scientific Data
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Background
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Key Product Details

Source

E. coli

Accession #

Q96PD4

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-17F Proteins and Enzymes »

Product Specifications

Description

A disulfide-linked homodimer of two 134 amino acid polypeptide chains corresponding to IL-17F Source: E. coli

Uniprot ID: Q96PD4

Amino Acid Sequence: MRKIPKVGHT FFQKPESCPP VPGGSMKLDI GIINENQRVS MSRNIESRST SPWNYTVTWD PNRYPSEVVQ AQCRNLGCIN AQGKEDISMN SVPIQQETLV VRRKHQGCSV SFQLEKVLVT VGCTCVTPVI HHVQ

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-17F as determined by LAL method.

Predicted Molecular Mass

30.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL-17F protein is fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 20 ng/ml, corresponding to a specific activity of > 5.0 x 10^4 IU/mg

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IL-17F Protein

SDS-PAGE: Recombinant Human IL-17F Protein [NBP2-35046]

SDS-Page: Human IL-17F Protein [NBP2-35046]

Formulation, Preparation and Storage

NBP2-35046

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in 6 mM HCl to a concentration of 0.1mg/ml. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-17F

Human Interleukin-17F (IL-17F) is encoded by the IL17F gene located on the chromosome 6 and belongs to the IL-17 family which contains IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17F that shares 50 % homologous of crystal structure to IL-17A and is expressed by activated T cells, has the functions of stimulating production of other cytokines (such as IL-6, IL-8 and granulocyte colony-stimulating factor) and proliferation of PBMC and T-cell, regulating cartilage matrix turnover, and inhibiting angiogenesis.

Long Name

Interleukin 17F

Alternate Names

IL17F

Gene Symbol