Recombinant Human IL-19 Protein

Name: Recombinant Human IL-19 Protein
Brand: Novus Biologicals
SKU: NBP2-35047

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35047

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Product Datasheet / COA

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9UHD0

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-19 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 153 amino acids corresponding to IL-19 Source: E. coli

Uniprot ID: Q9UHD0

Amino Acid Sequence: LRRCLISTDM HHIEESFQEI KRAIQAKDTF PNVTILSTLE TLQIIKPLDV CCVTKNLLAF YVDRVFKDHQ EPNPKILRKI SSIANSFLYM QKTLRQCQEQ RQCHCRQEAT NATRVIHDNY DQLEVHAAAI KSLGELDVFL AWINKNHEVM SSA

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-19 as determined by LAL method.

Predicted Molecular Mass

17.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL-19 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20R alpha and human IL-20R beta co-transfected murine BaF3 pro-B cells is less than 1.5 ng/ml, corresponding to a specific activity of > 6.7 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35047

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in 100 mM HAC to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-19

Human Interleukin-19 (IL-19) is encoded by the IL19 gene, which is located on the chromosome 1. The protein belongs to the IL-10 family that includes IL-10, IL-20, IL-22, IL-24, and IL-26. As a monomer made of seven amphipathic helices, IL-19 has a helical bundle and shares the same cell surface receptor (IL-20R) with IL-20 and IL-24. It may play some important roles in inflammatory responses because it up-regulates IL-6 and TNF-alpha and induces apoptosis.

Long Name

Interleukin 19

Alternate Names

IL19

Gene Symbol