Recombinant Rat IL-2 Protein

Name: Recombinant Rat IL-2 Protein
Brand: Novus Biologicals
SKU: NBP2-35230

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35230

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Product Specifications
Scientific Data
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Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

P17108

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-2 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 135 amino acids corresponding to IL-2 Source: E. coli

Uniprot ID: P17108

Amino Acid Sequence: APTSSPAKET QQHLEQLLLD LQVLLRGIDN YKNLKLPMML TFKFYLPKQA TELKHLQCLE NELGALQRVL DLTQSKSFHL EDAGNFISNI RVTVVKLKGS ENKFECQFDD EPATVVEFLR RWIAICQSII STMTQ

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-2 as determined by LAL method.

Predicted Molecular Mass

15.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL2 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat IL-2 Protein

SDS-PAGE: Recombinant Rat IL-2 Protein [NBP2-35230]

SDS-Page: Rat IL-2 Protein [NBP2-35230]

Formulation, Preparation and Storage

NBP2-35230

Formulation	Lyophilized from a 0.2 um filtered concentrated sterile solution in 10 mM sodium citrate, pH 4.0.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-2

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, gammadelta T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for Tcell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL2Ralpha, 75 kDa IL2Rbeta, 64 kDa common gamma chain gammac/IL2Rgamma) that are present on the cell surface in varying preformed complexes. Recombinant rat IL-2 is a 15.3 kDa protein containing 134 amino acid residues and it shares about 82 %, 66 %, 66 %, 60 % and 54 % amino acid sequence identity with mouse, human, rhesus macaque, pig and equus caballus IL- 2, respectively.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Gene Symbol

IL2

UniProt