Would you please help confirm if NBP2-35231 can be applied in cell culture?

Name: Recombinant Porcine IL-2 Protein
Brand: Novus Biologicals
SKU: NBP2-35231

The recombinant porcine IL2 protein (NBP2-35231), which you were interested in, was produced in E. coli and highly purified away from all impurities, including endotoxin. This product is biologically active and therefore can be used with live cells. And the major concern of any reagent supplying to live cells is the presence of endotoxin. We have tested and thus controlled the endotoxin level to less than 1 EU/ug of endotoxin as determined by LAL method. You should be able to use NBP2-35231 in cell cultures to elicit the biological functions of interests.

Recombinant Porcine IL-2 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35231

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P26891

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-2 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 134 amino acids corresponding to IL-2 Source: E. coli

Uniprot ID: P26891

Amino Acid Sequence: APTSSSTKNT KKQLEPLLLD LQLLLKEVKN YENADLSRML TFKFYMPKQA TELKHLQCLV EELKALEGVL NLGQSKNSDS ANIKESMNNI NVTVLELKGS ETSFKCEYDD ETVTAVEFLN KWITFCQSIY STLT

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-2 as determined by LAL method.

Predicted Molecular Mass

15.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL2 Proteinis fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Porcine IL-2 Protein

SDS-PAGE: Recombinant Porcine IL-2 Protein [NBP2-35231]

SDS-Page: Porcine IL-2 Protein [NBP2-35231]

Formulation, Preparation and Storage

NBP2-35231

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-2

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, gamma delta, T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL2R alpha, 75 kDa IL2R beta, 64 kDa common gamma chain gamme c/IL2R gamma) that are present on the cell surface in varying preformed complexes. Recombinant porcine IL-2 is a 15.3 kDa protein containing 134 amino acid residues and it shares about 72 % amino acid sequence identity with mouse, human and rat IL-2. It also shares 60 % and 67 % acid sequence identity with rhesus macaque and equus caballus IL-2, respectively.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Gene Symbol

IL2

UniProt