Recombinant Human IL-2 [ Cys Ser125]Protein

Name: Recombinant Human IL-2 [ Cys Ser125]Protein
Brand: Novus Biologicals
SKU: NBP3-07100

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07100

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-2 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 133 amino acids corresponding to IL-2 Source: E. coli

Amino Acid Sequence: MPTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFSQSIIS TLT

Modification

Cys Ser125

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1.0 EU/ug of IL-2 as determined by LAL method.

Predicted Molecular Mass

15.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 x 10^7 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IL-2 [ Cys Ser125]Protein

SDS-PAGE: Recombinant Human IL-2 [ Cys Ser125]Protein [NBP3-07100]

SDS-Page: Recombinant Human IL-2 [ Cys Ser125]Protein [NBP3-07100] - Western blot under reducing and non-reducing conditions

Formulation, Preparation and Storage

NBP3-07100

Formulation	Lyophilized from a 0.2 mm filtered concentrated solution in 20 mM PB (pH 3.5).
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-2

Interleukin 2 (IL-2) is a potent immunoregulatory growth factor produced by T-cells following stimulation by mitogens or allogens. IL-2 promotes growth and differentiation of various cells of the immune system, such as activated T-cells and B-cells, NK cells, lymphokine activated killer cells, monocytes and macrophages. It is essential for proper immune response, it may also be an important factor in the natural suppression of autoimmunity. IL2 may be of use as an anti-tumor agent in cancer therapy. Recombinant human IL2 is a 15.5 kDa protein, consisting of 134 amino acid residues. Human and mouse IL-2 share 56% aa sequence identity and exhibit cross-species activity.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Gene Symbol

IL2

Additional IL-2 Products

Product Documents for Recombinant Human IL-2 [ Cys Ser125]Protein

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COA

Product Specific Notices for Recombinant Human IL-2 [ Cys Ser125]Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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