IL-21 Antibody Blocking Peptide

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76740PEP

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-76740PEP
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

Synthetic

Conjugate

Unconjugated

Applications

Antibody Competition

View all IL-21 Proteins and Enzymes »

Product Specifications

Description

A blocking peptide containing 15 amino acids near the center of human IL-21.

Source: Synthetic

(Accession #: NP_068575)

Purity

N/A

Application Notes

This peptide is useful as a blocking peptide for NBP1-76740. For further blocking peptide related protocol, click here.

Protein / Peptide Type

Antibody Blocking Peptide

Scientific Data Images for IL-21 Antibody Blocking Peptide

Western Blot: IL-21 Antibody Blocking Peptide [NBP1-76740PEP]

Western Blot: IL-21 Antibody Blocking Peptide [NBP1-76740PEP]

Western Blot: IL21 Peptide [NBP1-76740PEP] - blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37C
IL-21 Antibody Blocking Peptide

Western Blot: IL-21 Antibody - BSA Free [NBP1-76740] -

Western Blot: IL-21 Antibody - BSA Free [NBP1-76740] - Figure 3 Western Blot Validation in Human HL-60 Cell LysateLoading: 15 u of lysates per lane.Antibodies: IL-21 (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.(A) Absence of blocking peptide(B) Presence of blocking peptide.
IL-21 Antibody Blocking Peptide

Western Blot: IL-21 Antibody - BSA Free [NBP1-76740] -

Western Blot: IL-21 Antibody - BSA Free [NBP1-76740] - Figure 3 Western Blot Validation in Human HL-60 Cell Lysate
Loading: 15 u of lysates per lane.Antibodies: IL-21 (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.(A) Absence of blocking peptide(B) Presence of blocking peptide.

Formulation, Preparation and Storage

NBP1-76740PEP
Formulation PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin.
Preservative 0.02% Sodium Azide
Concentration 0.2 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: IL-21

IL-21 (Interleukin-21) is member of the family of cytokines that utilize the common gamma chain as a receptor subunit. This subunit is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells. It exerts its biological effects through a heterodimeric receptor complex of the common gamma chain and the IL-21-specific IL-21 R. IL-21 supports Tfh cell development, immunoglobulin affinity maturation, plasma cell differentiation, B cell memory responses, and the migration of dendritic cells to draining lymph nodes. It costimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization. It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells. In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation.

Long Name

Interleukin 21

Alternate Names

CVID11, IL21, Za11

Gene Symbol

IL21

Additional IL-21 Products

Product Documents for IL-21 Antibody Blocking Peptide

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-21 Antibody Blocking Peptide

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.