Recombinant Mouse IL-28B/IFN-lambda 3 Protein

Name: Recombinant Mouse IL-28B/IFN-lambda 3 Protein
Brand: Novus Biologicals
SKU: NBP2-35077

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35077

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Product Specifications
Scientific Data
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Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

Q8CGK6

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-28B/IFN-lambda 3 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 174 amino acids corresponding to IL-28B/IFN-lambda 3 Source: E. coli

Uniprot ID: Q8CGK6

Amino Acid Sequence: DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKGAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENINDS ALTTILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFQLL LRDLKCVASG DQCV

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-28B/IFN-lambda 3 as determined by LAL method.

Predicted Molecular Mass

19.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL-28B/IFN-lambda 3 protein is fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 30 ng/ml, corresponding to a specific activity of > 3.3 x 10^4 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse IL-28B/IFN-lambda 3 Protein

SDS-PAGE: Recombinant Mouse IL-28B/IFN-lambda 3 Protein [NBP2-35077]

SDS-Page: Recombinant Mouse IL-28B/IFN-lambda 3 Protein [NBP2-35077] - Mouse IL-28B/IFN-lambda 3 Protein [NBP2-35077]

Formulation, Preparation and Storage

NBP2-35077

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-28B/IFN-lambda 3

Interferon lambda 3 (IFN-lambda3), also named as interleukin 28B (IL28B), is a protein encoded by the IL28B gene. IL28B, IL28A and IL29 are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by these three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor beta (IL-10Rbeta) and interleukin 28 receptor alpha (IL-28Ralpha). IFN-lambda3 is a cytokine with immune-modulatory activity. It up regulates MHC class I antigen expression and displays potent antiviral activity and antitumor activity, also plays an important role in Toll-like receptor (TLR)- induced antiviral defense and in the antiviral immune defense in the intestinal epithelium. In individuals with chronic hepatitis C, the presence of specific polymorphisms near this gene has been associated with an increased response in treatment for the condition with interferon and ribavirin. The same polymorphisms were also demonstrated at higher frequencies in individuals infected with hepatitis C who cleared the infection spontaneously, compared to those that developed chronic hepatitis from the condition. Murine IFN-lambda3 shares 63 % amino acid sequence identity with human IFN-lambda3.

Long Name

Interleukin 28B

Alternate Names

IFN-lambda 3, IFN-lambda 3/IL-28B

Gene Symbol

IFNL3

UniProt