Recombinant Mouse IL-33 Protein

Name: Recombinant Mouse IL-33 Protein
Brand: Novus Biologicals
SKU: NBP2-35124

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35124

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Product Specifications
Scientific Data
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Background
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Key Product Details

Source

E. coli

Accession #

Q8BVZ5

Conjugate

Unconjugated

Applications

Bioactivity, Flow Cytometry, SDS-PAGE

View all IL-33 Proteins and Enzymes »

Product Specifications

Description

A protein containing 158 amino acid residues corresponding to IL-33 Source: E. coli

Uniprot ID: Q8BVZ5

Amino Acid Sequence: SIQGTSLLTQ SPASLSTYND QSVSFVLENG CYVINVDDSG KDQEQDQVLL RYYESPCPAS QSGDGVDGKK LMVNMSPIKD TDIWLHANDK DYSVELQRGD VSPPEQAFFV LHKKSSDFVS FECKNLPGTY IGVKDNQLAL VEEKDESCNN IMFKLSKI

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-33 as determined by LAL method.

Predicted Molecular Mass

17.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL33 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg.

Application Notes

Use in Flow reported in scientific literature (PMID:35874724).

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse IL-33 Protein

SDS-PAGE: Recombinant Mouse IL-33 Protein [NBP2-35124]

SDS-Page: Mouse IL-33 Protein [NBP2-35124]

Formulation, Preparation and Storage

NBP2-35124

Formulation	Lyophilized from a 0.2 um filtered solution in PBS, and 1 mM EDTA.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-33

IL-33 is a number of IL-1 superfamily, secreted by high endothelial venules at high levels, which is found in tonsils, peyer patches and mesenteric lymph nodes, but not in placenta. It elicits its biological effects by interacting with IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-33 induces production of TH2 cell related cytokines, including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities. Mature IL-33 share approximately 55 % and 90 % a.a. sequence identity with human and rat IL-33 respectively.

Long Name

Interleukin 33

Alternate Names

C9orf26, DVS27, IL33, NF-HEV

Gene Symbol

IL33

UniProt