Recombinant Mouse IL-36 alpha/IL-1F6 Protein

Name: Recombinant Mouse IL-36 alpha/IL-1F6 Protein
Brand: Novus Biologicals
SKU: NBP2-35127

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35127

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Product Specifications
Scientific Data
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Key Product Details

Source

E. coli

Accession #

Q9JLA2

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-36 alpha/IL-1F6 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 153 amino acids corresponding to IL-36 alpha/IL-1F6 Source: E. coli

Amino Acid Sequence: RAASPSLRHV QDLSSRVWIL QNNILTAVPR KEQTVPVTIT LLPCQYLDTL ETNRGDPTYM GVQRPMSCLF CTKDGEQPVL QLGEGNIMEM YNKKEPVKAS LFYHKKSGTT STFESAAFPG WFIAVCSKGS CPLILTQELG EIFITDFEMI VVH

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-36 alpha/IL-1F6 as determined by LAL method.

Predicted Molecular Mass

17.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL36 alpha Protein is fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 25 ng/ml, corresponding to a specific activity of > 4.0 x 10^4 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse IL-36 alpha/IL-1F6 Protein

SDS-PAGE: Recombinant Mouse IL-36 alpha/IL-1F6 Protein [NBP2-35127]

SDS-Page: Mouse IL-36 alpha/IL-1F6 153 a.a Protein [NBP2-35127]

Formulation, Preparation and Storage

NBP2-35127

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, 1 mM DTT, 3 % trehalose.
Preservative	Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-36 alpha/IL-1F6

Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36alpha, IL-36beta, and IL-36gamma (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36alpha is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. It is expressed by monocytes, B and T cells. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. Recombinant murine interleukin-36 alpha contains 153 amino acids residues which is a single nonglycosylated polypeptide. Specifically, mouse IL-36alpha shares 83 % a.a. sequence identity with rat IL-36alpha, 54 - 60 % with human, rabbit, equine and bovine IL-36alpha.

Long Name

Interleukin 36 alpha/Interleukin 1 Family 6

Alternate Names

FIL1 epsilon, IL-1E, IL1F6, IL36 alpha, IL36A

Gene Symbol

IL36A

UniProt