Recombinant Mouse IL-36 alpha/IL-1F6 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35127
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 153 amino acids corresponding to IL-36 alpha/IL-1F6 Source: E. coli
Amino Acid Sequence: RAASPSLRHV QDLSSRVWIL QNNILTAVPR KEQTVPVTIT LLPCQYLDTL ETNRGDPTYM GVQRPMSCLF CTKDGEQPVL QLGEGNIMEM YNKKEPVKAS LFYHKKSGTT STFESAAFPG WFIAVCSKGS CPLILTQELG EIFITDFEMI VVH
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of IL-36 alpha/IL-1F6 as determined by LAL method.
Predicted Molecular Mass
17.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
IL36 alpha Protein is fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 25 ng/ml, corresponding to a specific activity of > 4.0 x 10^4 IU/mg.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Mouse IL-36 alpha/IL-1F6 Protein
![SDS-PAGE: Recombinant Mouse IL-36 alpha/IL-1F6 Protein [NBP2-35127]](https://resources.bio-techne.com/images/products/Mouse-IL-36-alpha-IL-1F6-153-a.a-Protein-SDS-Page-NBP2-35127-img0001.jpg "SDS-PAGE: Recombinant Mouse IL-36 alpha/IL-1F6 Protein [NBP2-35127]")
SDS-PAGE: Recombinant Mouse IL-36 alpha/IL-1F6 Protein [NBP2-35127]
SDS-Page: Mouse IL-36 alpha/IL-1F6 153 a.a Protein [NBP2-35127]Formulation, Preparation and Storage
NBP2-35127
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, 1 mM DTT, 3 % trehalose. |
| Preservative | Trehalose |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: IL-36 alpha/IL-1F6
Long Name
Interleukin 36 alpha/Interleukin 1 Family 6
Alternate Names
FIL1 epsilon, IL-1E, IL1F6, IL36 alpha, IL36A
Gene Symbol
IL36A
UniProt
Additional IL-36 alpha/IL-1F6 Products
Product Documents for Recombinant Mouse IL-36 alpha/IL-1F6 Protein
Product Specific Notices for Recombinant Mouse IL-36 alpha/IL-1F6 Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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