Recombinant Human IL-36 alpha/IL-1F6 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34876
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 153 amino acids corresponding to IL-36 alpha/IL-1F6 Source: E. coli
Uniprot ID: Q9UHA7
Amino Acid Sequence: KIDTPQQGSI QDINHRVWVL QDQTLIAVPR KDRMSPVTIA LISCRHVETL EKDRGNPIYL GLNGLNLCLM CAKVGDQPTL QLKEKDIMDL YNQPEPVKSF LFYHSQSGRN STFESVAFPG WFIAVSSEGG CPLILTQELG KANTTDFGLT MLF
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of IL-36 alpha/IL-1F6 as determined by LAL method.
Predicted Molecular Mass
17.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
IL36 alpha Protein is fully biologically active when compared to standard. The ED50 as determined by inducing IL-8 secretion in human preadipocytes is less than 10 ng/ml, corresponding to a specific activity of > 1.0 x 10^5 IU/mg.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human IL-36 alpha/IL-1F6 Protein
![SDS-PAGE: Recombinant Human IL-36 alpha/IL-1F6 Protein [NBP2-34876]](https://resources.bio-techne.com/images/products/Recombinant-Human-IL-36-alpha-IL-1F6-Protein-SDS-Page-NBP2-34876-img0002.jpg "SDS-PAGE: Recombinant Human IL-36 alpha/IL-1F6 Protein [NBP2-34876]")
SDS-PAGE: Recombinant Human IL-36 alpha/IL-1F6 Protein [NBP2-34876]
SDS-Page: Recombinant Human IL-36 alpha/IL-1F6 Protein [NBP2-34876]Formulation, Preparation and Storage
NBP2-34876
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.0, 0.1 % Tween 80. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: IL-36 alpha/IL-1F6
Long Name
Interleukin 36 alpha/Interleukin 1 Family 6
Alternate Names
FIL1 epsilon, IL-1E, IL1F6, IL36 alpha, IL36A
Gene Symbol
IL36A
UniProt
Additional IL-36 alpha/IL-1F6 Products
Product Documents for Recombinant Human IL-36 alpha/IL-1F6 Protein
Product Specific Notices for Recombinant Human IL-36 alpha/IL-1F6 Protein
This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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